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Clentbuterol hydrochloride is a substance commonly used in veterinary medicine as a bronchodilator. However, due to its anabolic effects, it has also found its way into the world of sports as a performance-enhancing drug. The use of clenbuterol hydrochloride by athletes is prohibited by the World Anti-Doping Agency (WADA) and other sports organizations, as it can lead to adverse health effects and distort the level playing field of sports.
Due to its misuse in sports, there is a growing need for accurate and precise analytical methods to detect clenbuterol hydrochloride’s presence in biological samples, such as urine and blood. The development of such assays can aid in enforcing anti-doping rules and regulations and maintaining fair play in sports.
Various analytical methods have been developed over the years, including chromatography, immunoassays, and mass spectrometry, with varying degrees of success in detecting clenbuterol hydrochloride in different biological matrices. The accurate determination of clenbuterol hydrochloride concentrations in biological samples is essential, as even minor deviations can impact the validity of test results and lead to unjustified penalties.
This article aims to highlight the different analytical methods used for clenbuterol hydrochloride testing, their strengths and limitations, and the processing and interpretation of assay results. By doing so, this article intends to contribute to the growing body of knowledge in the field of anti-doping and assist researchers and analysts in developing more precise and reliable testing techniques.
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Abstract The use of clenbuterol (CLE) as a growth-promoting agent in livestock is banned in the USA, European Union, and Mexico. Nevertheless, different studies have reported the presence of this agent in meat products, potentially causing intoxication in consumers. Electrochemical detection of clenbuterol with gold-nanoparticles-modified porous boron-doped diamond electrode. Immunochromatographic fluorometric determination of clenbuterol with enhanced sensitivity. Immunochromatographic assay using brightly colored silica nanoparticles as visible label for point-of-care detection of clenbuterol. Clenbuterol (Broncodil and trade) is a direct-acting sympathomimetic agent with mainly beta-adrenergic activity and a selective action on β2 receptors (a β2 agonist). It has properties similar to those of salbutamol. Clenbuterol (Broncodil and trade) is a direct-acting sympathomimetic agent with mainly beta-adrenergic activity and a selective action on β2 receptors (a β2 agonist). It has properties similar to. Clenbuterol is a sympathomimetic amine used by sufferers of breathing disorders as a decongestant and bronchodilator. People with chronic breathing disorders such as asthma use this as a bronchodilator to make breathing easier. It is most commonly available as the hydrochloride salt, clenbuterol hydrochloride. Clenbuterol (Broncodil and trade) is a direct-acting sympathomimetic agent with mainly beta-adrenergic activity and a selective action on β2 receptors (a β2 agonist). It has properties similar to those of salbutamol. Protein concentrations of UGTs and microsomes were determined with a BCA Protein Assay Kit (Pierce Chemical, Rockford, IL, USA). Synthesis of clenbuterol glucuronidesClenbuterol hydrochloride (233 mg, 0. 742 mmol) was dissolved in water (10 ml), and the pH of the solution was adjusted to 10 with 1 M NaOH aqueous. A highly sensitive fluorescent probe for clenbuterol hydrochloride (CLB) detection has been first designed based on its catalytic effect on NaIO4 oxidating eosine Y (R). And this environment-friendly, simple, rapid, selective and sensitive fluorescent probe has been utilized to detect CLB in the practical samples with the results consisting with those obtained by GC/MS. This research outlines the application of an enzyme-linked immunosorbent assay (ELISA) for the analysis of clenbuterol in animal products. Our assay showed good sensitivity for clenbuterol (0. 4 ng/g or 0. 4 ppb) and low detection limit (0. 09 ng/g or 0
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